[1]王海峰,包木太,韩红,等.一株枯草芽孢杆菌分离鉴定及其降解稠油特性[J].深圳大学学报理工版,2009,26(3):221-227.
 WANG Hai-feng,BAO Mu-tai,HAN Hong,et al.Isolation and identification of a Bacillus subtilis and its characterization in degrading heavy oil[J].Journal of Shenzhen University Science and Engineering,2009,26(3):221-227.
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一株枯草芽孢杆菌分离鉴定及其降解稠油特性()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第26卷
期数:
2009年3期
页码:
221-227
栏目:
环境与能源
出版日期:
2009-07-31

文章信息/Info

Title:
Isolation and identification of a Bacillus subtilis
and its characterization in degrading heavy oil
文章编号:
1000-2618(2009)03-0221-07
作者:
王海峰1包木太1韩红2李希明3王江涛1李阳4
1)海洋化学理论与工程技术教育部重点实验室,中国海洋大学,青岛 266100;
2)聊城大学图书馆,山东 聊城 252059;
3)中国石油化工股份有限公司胜利油田分公司采油工艺研究院,山东 东营 257000;
4)中国石油化工股份有限公司油田勘探事业开发部,北京 100029
Author(s):
WANG Hai-feng1BAO Mu-tai1HAN Hong2LI Xi-ming3WANG Jiang-tao1and LI Yang4
1)Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,Ocean University of China,Qingdao 266100,P.R. China
2)Liaocheng University Library,Liaocheng 252059,Shandong,P.R. China
3)Research Institute of Oil Production Technology,Shengli Oilfield Filiale,Sinopec,Dongying 257000,Shandong,P.R. China
4)Oilfield Exploitation Department,Sinopec,Beijing 100029,P.R. China
关键词:
石油烃降解菌生物降解枯草芽孢杆菌气相色谱/质谱分析16S rDNA
Keywords:
hydrocarbon degradation bacteriabiodeqradationbacillus subtilisgas chromatography mass spectrometry(GC-MS)16S rDNA
分类号:
Q 93-331; X 741; X 703.1
文献标志码:
A
摘要:
以稠油为唯一碳源,从被稠油污染过的土壤中筛选到一株高效石油烃降解菌,经生理生化鉴定和16S rDNA鉴定确认其为枯草芽孢杆菌.在摇瓶实验中,该菌最佳降解温度为35~45 ℃,最佳pH值为7.5~8.5,最佳盐质量浓度为8 ~16 g/L.在最佳降解条件下,当油质量浓度为0.1 g/L时,稠油降解率达34.3%.利用GC-FID分析知,该菌主要降解稠油中n-C9~n-C40的烷烃组分;利用GC-MS分析得知,该菌对萘及烷基化萘去除彻底,对二苯并噻吩、芴和稠二萘等部分芳烃类化合物有降解作用,在稠油降解过程中菲及菲的衍生物有所增加.
Abstract:
Heavy oil was used as the sole carbon source to isolate and screen for bacteria degrading petroleum hydrocarbon from the heavy oil polluted soil.The strain CZ-1 was isolated and identified as Bacillus subtilis by physiological and biochemical identification and analysis of 16S rDNA.Shaking culture experiment results show that the best degradation conditions of the strain CZ-1 were pH 7.5~8.5,35~45 ℃,and the mineralized rate 8 ~16 g/L.When the heavy oil density is 0.1 g/L,the degrading rate of heavy oil is up to 34.3% in the optimal conditions.GC-FID analysis indicates that in the saturate hydrocarbons,n-alkane could be degraded significantly;GC-MS analysis indicates that in the aromatic hydrocarbons,naphthalene and alkyl naphthalene almost disappeared after degradation,dibenzothiophene were degraded significantly by the strain CZ-1,while phenanthrene and alkyl phenanthrene increased.

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备注/Memo

备注/Memo:
收稿日期:2008-09-23;修回日期:2009-04-20
基金项目:国家自然科学基金资助项目(50604013);国家科技支撑计划“十一五”资助项目(2006BAB04B02)
作者简介:王海峰(1977-),男(汉族),山东省东营市人,中国海洋大学博士研究生.E-mail:317238920@qq.com
通讯作者:包木太(1971-),男(汉族),中国海洋大学教授、博士生导师.E-mail:mtbao@mail.ouc.edu.cn
更新日期/Last Update: 2009-08-02