[1]王华英,刘景波,张亦卓,等.高分辨率数字全息相衬成像技术[J].深圳大学学报理工版,2008,25(4):381-386.
 WANG Hua-ying,LIU Jing-bo,ZHANG Yi-zhuo,et al.Digital holographic imaging technique for high resolution phase-contrast image[J].Journal of Shenzhen University Science and Engineering,2008,25(4):381-386.
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高分辨率数字全息相衬成像技术()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第25卷
期数:
2008年4期
页码:
381-386
栏目:
生物医学工程
出版日期:
2008-10-31

文章信息/Info

Title:
Digital holographic imaging technique for high resolution phase-contrast image
文章编号:
1000-2618(2008)04-0381-06
作者:
王华英12刘景波1张亦卓2王大勇2
1)河北工程大学理学院,邯郸 056038
2)北京工业大学应用数理学院,北京 100022
Author(s):
WANG Hua-ying12LIU Jing-bo1ZHANG Yi-zhuo2and WANG Da-yong2
1) College of Sciences,Hebei University of Engineering,Handan 056038,P.R.China
2)College of Applied Sciences,Beijing University of Technology,Beijing 100022,P.R.China
关键词:
数字全息技术分辨率显微成像畸变补偿相衬成像
Keywords:
digital holographyresolutionmicroscopic imagingaberration compensationphase-contrast image
分类号:
O 438.1
文献标志码:
A
摘要:
采用傅里叶频谱对数方法进行自动聚焦,建立一套显微数字全息记录光路.提出综合利用自动相位补偿程序和手动调节记录参量的方法,对强噪声干扰的全息图进行数值重建,得到美国空军分辨率测试板准确的相位像,实现横向大于2.38 μm、轴向不超过10 nm的成像分辨率.实验结果表明,在强噪声情况下,利用自动相位补偿方法只能得到粗略的记录参量;要获得准确的相位信息,必须手动精调.记录高质量数字全息图是数字全息技术的关键.
Abstract:
An experimental digital holographic microscopy was built. The focal plane was obtained automatically by Fourier spectrum method. A method combining automatic phase aberration compensation procedure with manual adjustment of the reconstruction parameters was proposed.This method reconstructed the digital hologram under strong noise. The accurate phase-contrast image of the test USAF target has been obtained. The lateral resolution was better than 2.38 μm and the axial resolution was less than 10 nm. The experimental results show that under strong noisy background, the automatic phase aberration compensation procedure must be combined with manual adjustment for accurate phase reconstruction. This indicates the important to record high quality digital hologram.

参考文献/References:

[1]Kemper B,Bally G.用于活体细胞检测的数字全息显微镜方法[J].应用光学,2008,47(4):A52-A61(英文版).
[2]Colomb T,Cuche E,Charrière F,等.用于数字全息显微畸变补偿和样品形状补偿的自动程序[J].应用光学,2006,45(5):851-863(英文版).
[3]Volker K,Hans J H,Jptner W.应用于微光组分检测的数字全息显微镜方法[J].SPIE,2001,4398:189-198(英文版).
[4]Udupa G,Ngoi B,Freddy H,等.基于数字全息术的非抛光的硅表面缺陷探测[J].测量科学与技术,2004,5(1):35-43(英文版).
[5]Novak E.微机电系统度量技术[J].SPIE,2005,5716:173-181(英文版).
[6]Coppola G,Iodice M,Finizio A,等.用于微电机系统表征和设计的数字全息显微技术[J].微印刷、微制造和微系统,2005,4(1):1-9(英文版).
[7]Liebling M M,Blu T,Cuche E,等 .一种新奇的、用于数字全息显微的非衍射重建方法[C]//IEEE 2002年第1届生物医学国际研讨会论文集-从宏观到微观(ISBI’02), 华盛顿: IEEE, 2002:625-628(英文版).
[8]Ferraro F,Alferi D,De Nicola S, 等.基于横行剪切方法的数字全息的定量的相衬显微成像[J].光学快报,2006,31(10):1405-1407(英文版).
[9]王华英, 王广俊, 赵洁, 等.数字全息显微系统的成像分辨率分析[J].中国激光, 2007, 34(12): 1670-1675.
[10] Langehanenberg P, Kemper B, Dirksen D,等. 用于生物细胞成像的数字全息相称显微的自动聚焦[J].应用光学, 2008, 47(19): D176-D185(英文版).


[1]Kemper B,Bally G.Digital holographic microscopy for live cell applications and technical inspection[J].Applied Optics,2008,47(4):A52-A61.
[2]Colomb T,Cuche E,Charrière F,et al.Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation[J].Applied Optics,2006,45(5),851-863.
[3]Volker K,Hans J H,Jptner W.Application of digital holographic microscopy for inspection of micro-optical components[J].SPIE,2001,4398:189-198.
[4]Udupa G,Ngoi B,Freddy H,et al.Defect detection in unpolished Si wafers by digital holography[J].Measurement Science and Technology,2004,5(1):35-43.
[5]Novak E.MEMS metrology techniques[J].SPIE,2005,5716:173-181.
[6]Coppola G,Iodice M,Finizio A, et al.Digital holography microscope as tool for microelectromechanical systems characterization and design[J].Journal of Microlithography, Microfabrication, and Microsystems,2005,4(1):1-9.
[7]Liebling M,Blu T,Cuche E, et al.A novel non-diffractive reconstruction method for digital holographic microscopy[C]//Proceedings of the First 2002 IEEE International Symposium on Biomedical Imaging,Macro to Nano(ISBI′02),Washington DC: IEEE, 2002:625-628.
[8]Ferraro P,Alferi D,De Nicola S, et al.Quantitative phase-contrast micros-copy by a lateral shear approach to digital holographic image reconstruction[J].Optics Letters,2006,31(10):1405-1407.
[9]WANG Hua-ying, WANG Guang-jun, ZHAO Jie, et al. Imaging resolution analysis of digital holographic microscopy[J].Chinese Journal of Lasers, 2007, 34(12): 1670-1675(in Chinese).
[10]Langehanenberg P, Kemper B, Dirksen D, et al. Autofocusing in digital holographic phase contrast microscopy on pure phase objects for life cell imaging[J].Appl Opt, 2008, 47(19): D176-D185.

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备注/Memo

备注/Memo:
收稿日期:2008-05-29
基金项目:国家自然科学基金资助项目(60577029);河北省自然科学基金资助项目(F2008000750)
作者简介:王华英(1963-),女(汉族),河北省涉县人,河北工程大学副教授、北京工业大学博士研究生.E-mail:pbxsyingzi@126.com
通讯作者:王大勇(1968-),男(汉族),北京工业大学教授、博士生导师.E-mail:wdyong@bjut.edu.cn
更新日期/Last Update: 2008-11-26