[1]陈丹妮,于斌,刘夏,等.基于数字微镜装置的结构光照明层析成像[J].深圳大学学报理工版,2008,25(4):376-380.
 CHEN Dan-ni,YU Bin,LIU Xia,et al.Sectioned imaging of DMD-based structured illumination fluorescence microscopy[J].Journal of Shenzhen University Science and Engineering,2008,25(4):376-380.
点击复制

基于数字微镜装置的结构光照明层析成像()
分享到:

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第25卷
期数:
2008年4期
页码:
376-380
栏目:
生物医学工程
出版日期:
2008-10-31

文章信息/Info

Title:
Sectioned imaging of DMD-based structured illumination fluorescence microscopy
文章编号:
1000-2618(2008)04-0376-05
作者:
陈丹妮12于斌2刘夏2屈军乐2牛憨笨2
1)华中科技大学光电子科学与工程学院,武汉430074
2)深圳大学光电子学研究所,光电子器件与系统教育部重点实验室,广东省光电子器件与系统重点实验室,深圳 518060
Author(s):
CHEN Dan-ni12YU Bin2LIU Xia2QU Jun-le2and Hanben NIU2
1)College of Optoelectronic Science and Engineering,Huazhong University of Science and Technology,Wuhan 430074,P.R.China
2)Institute of Optoelectronics Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education,Key Laboratory of Optoelectronics Devices and Systems of Guangdong Province,Shenzhen University,Shenzhen 518060,P.R.China
关键词:
结构光照明数字微镜层析成像分辨率
Keywords:
structured illumination microscopydigital micromirror devicesectioned imagingresolution
分类号:
TN 247
文献标志码:
A
摘要:
在结构光显微系统中使用可编程数字微镜装置代替压电驱动的光栅,产生稳定可靠的结构光照明.为避免数字微镜装置本身的像素化结构对成像的影响,系统中引入一个低通的空间光调制器,以滤除经过数字微镜装置后产生的高级(±2nd 及以上)衍射光.此时在样品上形成的结构光不再是单一频率的余弦光栅图案,传统结构光显微成像中通过光栅3步相移成像获得层析图像的方法不再适用.新算法通过5步相移得到5幅源图像,也可重构出层析图像.结果表明,传统荧光图像中几乎不能分辨的某些精细结构,在重构的层析图像中能够清晰地分辨出来.
Abstract:
In our novel structured illumination microscope (SIM),a programmable digital micromirror device (DMD) was used instead of piezo activated grating to produce stable and reliable structured illumination. An optical low-pass spatial filter was applied to block the diffraction order higher than ±1st order. The illumination pattern was not a sinusoidal grid with a single spatial frequency,so the algorithm used in the traditional SIM is not proper here. An improved algorithm based on the five raw images with different phase angles was introduced and discussed in detail.The results have demonstrated that the sectioned image can be reconstructed from five raw images with different phase angles,with resolving some fine lateral structure that was blurred in traditional microscopic image.

参考文献/References:

[1]Neil M,Jukaitis R,Wilson T.在传统显微镜中通过使用结构光照明获得光学层析的方法[J].光学快报,1997,22(24):1905-1907(英文版).
[2]Wilson T,Neil M A A,Jukaitis R.宽场荧光显微镜中的光学层析图像.[C]// 三维及多维显微技术:图像获取及处理V.华盛顿:SPIE,1998,3261:4-6(英文版).
[3]Hubert Bauch,Jrg Schaffer.通过结构光照明实现光学层析:背景及其在荧光显微技术当中的应用[J].Photomik国际,2006:86-88(英文版).
[4]Karadagli D,Jukaitis R,Wilson T.采用结构光照明的共聚焦内窥成像系统[J].扫描,2002,24:301-304(英文版).
[5]Tomasz S Tkaczyk,Mohammed Rahman,Vivian Mack,等.用结构光照明对光密组织进行分子特异性的高分辨反射成像[J].光学快讯,2004,12(16):3745-3758(英文版).
[6]Gustafsson M G L.用结构光照明显微实现横向超分辨[J].显微学报,2000,198:82-87(英文版).
[7]WONG C H,CHEN N G,Sheppard.C J R 采用数字微镜器件实现结构光照明显微内窥探讨[C]// 2006年生物光子学、纳米光学及超材料学会议论文集.纽约:IEEE,2006:218-221(英文版).

[1]Neil M,Jukaitis R,Wilson T.Method of obtaining optical sectioning by using structured light in a conventional microscope[J].Optics Letters,1997,22(24):1905-1907.
[2]Wilson T,Neil M A A,Jukaitis R.Optically sectioned images in widefield fluorescence microscopy[C]//Three-Dimensional and Multidimensional Microscopy:Image Aquisition and Processing V.Washington D C:SPIE,1998,3261:4-6.
[3]Hubert Bauch,Jrg Schaffer.Optical sections by means of structured illumination:background and application in fluorescence microscopy[J].Photonik International,2006:86-88.
[4]Karadagli D,Jukaitis R,Wilson T.Confocal endoscopy via structured illumination[J].Scanning,2002,24:301-304.
[5]Tomasz S Tkaczyk,Mohammed Rahman,Vivian Mack,et al.High resolution,molecular-specific,reflectance imaging in optically dense tissue phantoms with structured-illumination[J].Optics Express,2004,12(16):3745-3758.
[6]Gustaffson M G L.Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy[J].Journal of Microscopy,2000,198:82-87.
[7]Wong C H,Chen N G,Sheppard C J R.Study on potential of structured illumination microscopy utilizing digital micromirrordeviceforendoscopypurpose[C]//International Symposimosium on Biophotonics,Nanophotonics and Metamaterials 2006 Metamaterials. NY: IEEE, 2006: 218-221.

相似文献/References:

[1]牛憨笨,陈丹妮,尹君.细胞内分子检测及成像技术研究[J].深圳大学学报理工版,2011,28(No.1(001-095)):1.
 Han-ben NIU,CHEN Dan-ni,and YIN Jun.Advances in approaches of molecules detecting and imaging in cells[J].Journal of Shenzhen University Science and Engineering,2011,28(4):1.

备注/Memo

备注/Memo:
收稿日期:2008-09-09;修回日期:2008-09-21
基金项目:国家自然科学基金资助项目(60878053);广东省高等学校科技创新团队资助项目(06CXTD009);高等学校博士学科点专项科研基金资助项目(20070590001)
作者简介:陈丹妮(1976-), 女(汉族), 湖南省武冈市人, 深圳大学与华中科技大学联合培养博士研究生. E-mail: dannyc007@gmail.com
通讯作者:牛憨笨(1940-),男(汉族),中国工程院院士、深圳大学研究员.E-mail:hbniu@szu.edu.cn
*本文为2008第3届国际生物医学光学方法高级研讨会论文
更新日期/Last Update: 2008-11-26