[1]屈军乐,潘文良,赵伶羚,等.蟾酥灵诱导细胞内caspase-3活化特性荧光分析[J].深圳大学学报理工版,2008,25(3):244-247.
 QU Jun-le,PAN Wen-liang,ZHAO Ling-ling,et al.Analyzing caspase-3 activation induced by bufalin using fluorescence techniques in single living cell[J].Journal of Shenzhen University Science and Engineering,2008,25(3):244-247.
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蟾酥灵诱导细胞内caspase-3活化特性荧光分析()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第25卷
期数:
2008年3期
页码:
244-247
栏目:
生物医学工程
出版日期:
2008-07-31

文章信息/Info

Title:
Analyzing caspase-3 activation induced by bufalin using fluorescence techniques in single living cell
文章编号:
1000-2618(2008)03-0244-04
作者:
屈军乐1潘文良2赵伶羚1孙磊2王小平3陈同生2
1)深圳大学光电子学研究所,光电子器件与系统(教育部/广东省)重点实验室,深圳518060
2)华南师范大学激光生命科学研究所,激光生命科学教育部重点实验室,广州510631
3)暨南大学第一附属医院,广州510632
Author(s):
QU Jun-le1PAN Wen-liang2ZHAO Ling-ling1SUN Lei2WANG Xiao-ping3and CHEN Tong-sheng2
1)Institute of Optoelectronics,Key Laboratory of Optoelectronic Devices and Systems of  Ministry of Education and  Guangdong Province,Shenzhen University,Shenzhen 518060,P.R.China
2)Institute of Laser Life Science,MOE Key Laboratory of Laser Life Science,South China Normal University,Guangzhou 510631,P.R.China
3)The First Affiliated Hospital of Jinan University,Guangzhou 510632,P.R.China
关键词:
蟾酥灵荧光光谱荧光寿命成像caspase-3荧光共振能量转移
Keywords:
bufalinfluorescence spectrafluorescence lifetime imaging microscopy (FLIM)caspase-3fluorescence resonance energy transfer (FRET)
分类号:
Q 631
文献标志码:
A
摘要:
采用多光子激发荧光光谱和寿命成像显微技术,在单个活细胞内实时检测蟾酥灵(bufalin,Bu)诱导人类肺腺癌(ASTC-a-1)细胞死亡过程中caspase-3的活化特性.利用CCK-8(Cell Counting Kit-8)检测蟾酥灵对人类肺腺癌细胞活性的抑制效应.蟾酥灵处理稳定表达FRET质粒SCAT3的人类肺腺癌后,在不同时间点检测单个活细胞中SCAT3的多光子激发荧光光谱及其供体ECFP的荧光寿命,从而检测bufalin诱导细胞凋亡过程中caspase-3活化特性.实验结果表明:蟾酥灵以浓度依赖性的方式显著地抑制人类肺腺癌细胞的生长;蟾酥灵处理细胞24 h后,细胞内SCAT3未被切割,而蟾酥灵作用细胞48 h后SCAT3被切割.说明caspase-3参与调控蟾酥灵诱导人类肺腺癌细胞凋亡的过程.
Abstract:
Caspase-3 activation was analyzed inside single living cells during bufalin-induced human lung adenocarcinoma (ASTC-a-1) cell apoptosis by using multi-photon excitation spectra and fluorescence lifetime imaging microscopy (FLIM).CCK-8 was used to assay the inhibition of bufalin on the cells viability.The dynamical emission spectra of SCAT3 which is a fluorescence resonance energy transfer (FRET) plasmid, and fluorescence lifetime of ECFP were performed inside single living cells,stably expressing SCAT3 after bufalin treatment.Compared with controls,SCAT3 did not change after 24 h,but was cleaved after 48 h,which were verified by the changes of fluorescence spectra of SCAT3 and fluorescence lifetime of ECFP. Our data shows that the cells growth is significantly inhibited by bufalin in a dose-dependent manner;caspase-3 is involved in the bufalin- induced cell death.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2008-05-29
基金项目:国家自然科学基金资助项目(30670507,60378043,30470494)
作者简介:屈军乐(1970-),男(汉族),陕西省西安市人,深圳大学副研究员、博士.E-mail:jlqu@szu.edu.cn
通讯作者:陈同生(1964-),男(汉族),华南师范大学教授、博士生导师.E-mail:chentsh@scnu.edu.cn
*本文为2008年第3届国际生物医学光学方法高级研讨会宣读论文
更新日期/Last Update: 2008-08-07