[1]陈同生,卢盈颖,王龙祥,等.基于FRET效率分析细胞内caspase-3活化特性研究[J].深圳大学学报理工版,2008,25(3):233-237.
 CHEN Tong-sheng,LU Ying-ying,WANG Long-xiang,et al.Detection of caspase-3 activation by monitoring FRET efficiency in living cells[J].Journal of Shenzhen University Science and Engineering,2008,25(3):233-237.
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基于FRET效率分析细胞内caspase-3活化特性研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第25卷
期数:
2008年3期
页码:
233-237
栏目:
生物医学工程
出版日期:
2008-07-31

文章信息/Info

Title:
Detection of caspase-3 activation by monitoring FRET efficiency in living cells
文章编号:
1000-2618(2008)03-0233-05
作者:
陈同生1卢盈颖1王龙祥1屈军乐2
1)华南师范大学激光生命科学研究所,激光生命科学教育部重点实验室,广州510631
2)深圳大学光电子学研究所,光电子器件与系统(教育部/广东省)重点实验室,深圳518060
Author(s):
CHEN Tong-sheng1LU Ying-ying1WANG Long-xiang1and QU Jun-le2
1)Institute of Laser Life Science,MOE Key Laboratory of Laser Life Science,South China Normal University,Guangzhou 510631,P.R.China
2)Institute of Optoelectronics,Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Shenzhen University,Shenzhen 518060,P.R.China
关键词:
caspase-3荧光共振能量转移荧光光谱双氢青蒿素细胞凋亡
Keywords:
caspase-3fluorescence resonance energy transfer (FRET)fluorescence emission spectradihydroartemisinin(DHA)cell apoptosis
分类号:
Q 631
文献标志码:
A
摘要:
利用SCAT3在选择性激发CFP时的发射谱信息,通过光谱拟合定量获得FRET效率,监测双氢青蒿素诱导的人肺腺癌(ASTC-a-1)细胞内caspase-3的活化特性.实验结果表明:双氢青蒿素可有效抑制ASTC-a-1细胞的增殖并诱导细胞凋亡,且对细胞抑制呈剂量依赖性;双氢青蒿素诱导细胞中caspase-3的活化也呈剂量依赖性;通过光谱拟合得到的FRET效率表明,细胞中caspase-3活化程度越大,FRET效率越低,且FRET效率呈剂量依赖性递减.
Abstract:
A novel method to calculate FRET efficiency by fitting emission spectra of the donor-acceptor pair was used to detect DHA-induced caspase-3 activation in living cells stably expressed SCAT3.The results indicated that DHA effectively inhibited the proliferation of ASTC-a-1 cells via a concentration-dependent manner.Dose-dependent activation of caspase-3 was determined during the DHA-induced apoptosis.The decreased FRET efficiency was in response to the increased caspase-3 activities in DHA-treated cells.The report has proved,for the first time,that FRET efficiency is an appropriate method that can be used as a real time quantitative measurement of DHA-induced activation of caspase-3 in living cells.

参考文献/References:

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相似文献/References:

[1]屈军乐,潘文良,赵伶羚,等.蟾酥灵诱导细胞内caspase-3活化特性荧光分析[J].深圳大学学报理工版,2008,25(3):244.
 QU Jun-le,PAN Wen-liang,ZHAO Ling-ling,et al.Analyzing caspase-3 activation induced by bufalin using fluorescence techniques in single living cell[J].Journal of Shenzhen University Science and Engineering,2008,25(3):244.

备注/Memo

备注/Memo:
收稿日期:2008-05-29
基金项目:国家自然科学基金资助项目 (30670507,30470494,30627003)
作者简介:陈同生 (1964-), 男 (汉族), 湖北省武汉市人,华南师范大学教授、博士.E-mail:chentsh@scnu.edu.cn
*本文为2008年第3届国际生物医学光学方法高级研讨会宣读论文
更新日期/Last Update: 2008-08-07