[1]陈伟钊,刘森林,邢 苗.耐热碱性纤维素酶分离纯化及酶学特性研究[J].深圳大学学报理工版,2007,24(2):212-216.
 CHEN Wei-zhao,LIU Sen-lin,and XING Miao.Purification and enzymatic properties of thermostable alkaline cellulase Ⅲ-3-A[J].Journal of Shenzhen University Science and Engineering,2007,24(2):212-216.
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耐热碱性纤维素酶分离纯化及酶学特性研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第24卷
期数:
2007年2期
页码:
212-216
栏目:
生命科学
出版日期:
2007-04-30

文章信息/Info

Title:
Purification and enzymatic properties of thermostable alkaline cellulase Ⅲ-3-A
文章编号:
1000-2618(2007)02-0212-05
作者:
陈伟钊1刘森林1邢 苗12
1)深圳大学生命科学学院,深圳 518060;
2)华南农业大学,广州 510642
Author(s):
CHEN Wei-zhao1LIU Sen-lin1and XING Miao12
1)College of Life Science,Shenzhen University,Shenzhen 518060,P.R.China
2)South China Agricultural University,Guangzhou 510642,P. R. China
关键词:
芽孢杆菌碱性纤维素酶分离纯化离子交换层析凝胶过滤层析酶学性质
Keywords:
Bacillusalkaline cellulasepurificationion-exchangegel filtrationenzymatic properties
分类号:
Q 93
文献标志码:
A
摘要:
通过离子交换和凝胶过滤两步层析法,从耐碱芽孢杆菌Ⅲ-3的培养液中分离到相对分子质量约为89 kD、比活力高达420 U/mg的碱性纤维素酶Ⅲ-3-A.质谱分析显示:Ⅲ-3-A酶蛋白与芽孢杆菌KSM-64、KSM-S237等产生的A5家族碱性纤维素酶具有一定的同源性.酶学性质研究结果表明:Ⅲ-3-A的CMC酶反应以pH值为9.0、反应温度45℃左右为宜,在pH值为 6~11和50℃以下酶活性稳定,且具有耐金属离子和表面活性剂等特性;Ca2+对酶的最适反应温度和热稳定性有显著影响,添加5 m mol/L CaCl2可使最适酶反应温度和稳定性分别提高至50℃和55℃.该酶具有耐热、耐金属离子、耐碱及高比活力等特点,在棉织品的水洗整理及洗涤剂工业中具有良好应用前景.
Abstract:
Using a two-step column-chromatographic procedure of ion-exchange and gel filtration, alkaline cellulase Ⅲ-3-A, with molecular weight of about 89 kD and specific activity of 420 U/mg, was purified to homogeneity from the culture broth of Bacillus sp.Ⅲ-3.Result of MALDI-TOF mass spectrometry analysis showed that the typical amino acid sequences of Ⅲ-3-A were similar to those of alkaline cellulases from Bacillus sp.1139 and Bacillus sp.KSM-64, which belonged to family A5.The optimal conditions for the enzymatic reaction were about pH 9.0 at 45℃.At pH 6~11 and below 50℃, the enzyme showed high activity and good stability.The enzyme was resistant to most metallic ions and reagents explored.It was also found that Ca2+ ion had a significant influence on the optimal temperature for enzymatic reaction and thermostability.5 mmol/L CaCl2 lifted the optimum temperature and thermostability up to 50℃ and 55℃, respectively.This novel cellulase showed great potential in the textile biofinishing and detergent industry.

参考文献/References:

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相似文献/References:

[1]孔舒,刘刚,刘森林,等.ATCC碱性纤维素酶产生菌产酶特性的重新确定[J].深圳大学学报理工版,2005,22(3):253.
 KONG Shu,LIU Gang,LIU Sen-lin,et al.Restudy of the characters of alkaline cellulase production from an ATCC alkalophilic Bacillus strain[J].Journal of Shenzhen University Science and Engineering,2005,22(2):253.

备注/Memo

备注/Memo:
收稿日期:2006-11-14;修回日期:2007-02-08
基金项目:深圳市科技计划资助项目(200325);深圳大学科研启动基金资助项目(200714)
作者简介:陈伟钊(1983-),男(汉族),广东省惠来县人,深圳大学硕士研究生.E-mail:cwz757@21cn.com
通讯作者:邢 苗(1954-),男(汉族),华南农业大学教授、博士生导师. E-mail:xingmiao@scau.edu.cnxingmiao@szu.den.cn
更新日期/Last Update: 2007-12-09