[1]莫蓓莘.甘露糖外切酶的细胞生物学定位研究[J].深圳大学学报理工版,2006,23(4):358-361.
 MO Bei-xin.Possible subcellular localization of mannosidase[J].Journal of Shenzhen University Science and Engineering,2006,23(4):358-361.
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甘露糖外切酶的细胞生物学定位研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第23卷
期数:
2006年4期
页码:
358-361
栏目:
土木建筑工程
出版日期:
2006-10-30

文章信息/Info

Title:
Possible subcellular localization of mannosidase
文章编号:
1000-2618(2006)04-0358-04
作者:
莫蓓莘
深圳大学生命科学学院,深圳518060
Author(s):
MO Bei-xin
College of Life Science Shenzhen University Shenzhen 518060 P. R. China
关键词:
基因枪GFP洋葱表皮细胞甘露聚糖外切酶细胞壁
Keywords:
gene gunGFPonion epidermal cells fusion-proteincell wall
分类号:
Q 291
文献标志码:
A
摘要:
用不同试剂从西红柿种子提取甘露聚糖外切酶,结果表明甘露聚糖外切酶只能被含高盐的缓冲液(0.1mol/L Hepes+500mmol/L NaCl,pH7.0)提取出来,单独的低盐缓冲液(0.1mol/L Hepes,pH7.0)或用非盐洗涤剂代替高盐则不能提取该酶.通过基因枪将甘露聚糖外切酶和GFP融合蛋白转化洋葱表皮细胞,发现转化了甘露聚糖外切酶和GFP融合蛋白的洋葱表皮细胞壁上有荧光,表明甘露聚糖外切酶可能定位在细胞壁上.
Abstract:
β-mannosidase was extracted from tomato seeds with different buffers. The results show that the β-mannosidase activity is present in high salt buffer (0.1mo l /L Hepes + 500 mmol /L NaCl, pH 7.0), but not in low salt buffer. Non-ionic detergents can not replace the effect of high salt By introducing the β -mannosidase-GFP fusion protein construct into onion epidermal cells via gene gun bombardment, fluorescence can be observed in the β-mannosidase-GFP transformed onion cells. This indicat that β-mannosidase might be localized in the cell wall.
更新日期/Last Update: 2015-06-26