[1]叶小明,庾蕾,庄志雄,等.TaqMan-MGB探针检测人REV3L基因的毒物兴奋效应[J].深圳大学学报理工版,2005,22(4):358-363.
 YE Xiao-ming,YU Lei,ZHUANG Zhi-xiong,et al.Hormesis of human REV3L gene induced by low dose of UV detected by taqman-MGB probe[J].Journal of Shenzhen University Science and Engineering,2005,22(4):358-363.
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TaqMan-MGB探针检测人REV3L基因的毒物兴奋效应()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第22卷
期数:
2005年4期
页码:
358-363
栏目:
生命科学
出版日期:
2005-10-30

文章信息/Info

Title:
Hormesis of human REV3L gene induced by low dose of UV detected by taqman-MGB probe
文章编号:
1000-2618(2005)04-0358-06
作者:
叶小明12庾蕾1庄志雄1胡章立2
1)深圳市疾病预防控制中心,深圳518020
2)深圳大学生命科学学院,深圳518060
Author(s):
YE Xiao-ming1’2YU Lei1ZHUANG Zhi-xiong1HU Zhang-li2
1)Center for Disease Control and Prevention of Shenzhen,Shenzhen 518020 P. R. China
2)College of Life Science Shenzhen University Shenzhen 518060 P. R. China
关键词:
毒物兴奋效应紫外线REV3L TaqMan-MGB
Keywords:
hormesisultravioletREV3L TaqMan-MGB
分类号:
R 135. 1;Q 593.1
文献标志码:
A
摘要:
研究低剂量紫外线照射诱导人胚肺成纤维细胞( MRC-5 ) REV3L基因表达量变化的毒物兴奋效应,用四氮唑盐比色法(MTT法)检测细胞增值抑制率,得到紫外线对MRC-5毒性的剂量反应关系,并对低剂量处理组(10 s和20 s)和高剂量处理组(30 s, 60 s和300 s)染毒细胞,采用TaqMan-MGB探针,进行荧光实时定量PCR,检测REV3L基因表达量的变化.发现低剂量处理组(20 s)使REV3L基因的表达量明显增高.表明低剂量紫外线照射可以引起MRC-5细胞REV3L基因产生毒物兴奋效应.
Abstract:
The hormesis of the REV3L gene of human MRC-cells was induced by low doses of UV. The MTT method was used to detect the inhibition ratio of MRC-5 to obtain the dose response to UV. MRC-5 cells were treated with low doses(10 s、20 s) and high doses(30 s、60 s、300 s ) of UV and their response was measured by real time PCR using a Taqman-MGB probe. Results showed that the expression level of REV3L gene elevated following treatment by a low dose ( 20 s ) of UV. It indicates thaw low doses of UV radiation cause MRC-5 cells to generate the REV3L hormesis.
更新日期/Last Update: 2015-10-16