[1]刘士德,张建华,余少文,等.人胰岛素和胰岛素原单克隆抗体制备及分析[J].深圳大学学报理工版,2005,22(4):340-344.
　LIU Shi-de,ZHANG Jian-hua,YU Shao-wen,et al.The preparation and speciality analysis of monoclonal antibody to insulin and pro-insulin[J].Journal of Shenzhen University Science and Engineering,2005,22(4):340-344.
点击复制

人胰岛素和胰岛素原单克隆抗体制备及分析()
分享到：

《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第22卷
期数:
2005年4期
页码:
340-344
栏目:
生命科学
出版日期:
2005-10-30

文章信息/Info

Title:
The preparation and speciality analysis of monoclonal antibody to insulin and pro-insulin
文章编号:
1000-2618(2005)04-0340-05
作者:
刘士德; 张建华; 余少文; 张维德; 邢苗
深圳大学生命科学学院，深圳市微生物基因工程重点实验室，深圳518060
Author(s):
LIU Shi-de; ZHANG Jian-hua; YU Shao-wen ; ZHANG Wei-de; XING Miao
Shenzhen Key Laboratory of Microbial Gene Engineering College of Life Science Shenzhen University Shenzhen 518060 P. R.China
关键词:
胰岛素; 胰岛素原; 单克隆抗体
Keywords:
insulin; pro-insulin; monoclonal antibody(McAb)
分类号:
R 392
文献标志码:
A
摘要:
应用杂交瘤技术获得针对胰岛素和胰岛素原的各4株稳定分泌单克隆抗体的杂交瘤细胞.其中4株分泌胰岛素单克隆抗体的细胞( N_I_1、N-I-7 、N-I-13 、N-I-15)所分泌的抗体亚型均为IgG1;分泌胰岛素原单克隆抗体的细胞株所分泌的抗体亚型分别为IgA( N-PI-1) , IgG1 ( N-PI-3 , N-PI-5)和IgG2a( N-PI-9 );所有抗体的轻链均为K型.所制备的胰岛素原单克隆抗体均不与胰岛素和C肽交叉反应，但所有胰岛素单克隆抗体均与胰岛素原交叉反应.
Abstract:
Four hybrid cell lines which stably secret the McAb to insulin and four hybrid cell lines which stably secret McAb to pro-insulin were established. The subspecies of the McAbs secreted by the four cell lines(N-I-1、N-I-7、N-I-13、N-I-15)to insulin are IgG1, and those of the McAbs secreted by the four cell. lines to pro-insulin are IgA(N-PI-1)，IgG1(N-PI-3，N-PI-5) and IgG2a(N-PI-9). None of the McAbs of pro-insulin cross-react with insulin and C-peptide，while all the McAbs of insulin cross-react with pro-insulin.

相似文献/References:

[1]王静凤,傅佳,徐雷雷,等.日本刺参降血糖及对胰岛素信号通路的影响[J].深圳大学学报理工版,2011,28(No.2(095-188)):172.
　WANG Jing-feng,FU Jia,XU Lei-lei,et al.Involvement of the PI3K/Akt pathway in the hypoglycemic effects of sea cucumber Apostichopus japonicas in diabetic rats[J].Journal of Shenzhen University Science and Engineering,2011,28(4):172.
[2]胡世伟,王静凤,徐雷雷,等.海参磷脂型二十碳五烯酸降血糖机制研究[J].深圳大学学报理工版,2013,30(No.4(331-440)):349.[doi:10.3724/SP.J.1249.2013.04349]
　Hu Shiwei,Wang Jingfeng,Xu Leilei,et al.Mechanism of eicosapentaenoic acid-enriched phosphatidylcholine isolated from sea cucumber on anti-hyperglycemia[J].Journal of Shenzhen University Science and Engineering,2013,30(4):349.[doi:10.3724/SP.J.1249.2013.04349]

更新日期/Last Update: 2015-10-16