ATCC碱性纤维素酶产生菌产酶特性的重新确定-《深圳大学学报理工版》

文章信息/Info

Title:: Restudy of the characters of alkaline cellulase production from an ATCC alkalophilic Bacillus strain

Author(s):: KONG Shu; LIU Gang; LIU Sen-lin; XING Miao; College of Life Science Shenzhen University Shenzhen 518060 P. R.China

摘要:: 研究美国典型培养物保藏中心(ATCC)提供的菌株ATCC 21833 ( Horikoshi K定名为芽孢杆菌Bacillus sp. N-4 )的纤维素酶合成基因后，发现该基因与Horikoshi K定名的N-4基因并不具有同源性.为此重新研究了该菌株的产酶特征和所产生碱性纤维素酶的部分酶学特性.结果表明，羧甲基纤维素为最适宜的碳源，在以羧甲基纤维素、蛋白陈和酵母抽提物为主要组分的培养基中进行培养，碱性纤维素酶的活性可以达到0. 64 U/mL，所产生的碱性纤维素的最适pH值为10. 0，最适反应温度为40℃.其产酶特性和酶学性质和N-4菌株有较大差异.

Abstract:: We studied the alkaline cellulase gene of American Type Culture Collection(ATCC) stored strain ATCC 21833, which was reported to be Bacillus sp. N-4. The alkaline cellulase gene initially isolated from this strain showed low homology to the reported N-4 alkaline cellulase gene. Therefore we restudied the characters of alkaline cellulase production from this strain and characterized the produced cellulase. The results show that carboxymethylcellulose(CMC)was the best carbon source for the ATCC strain, and its alkaline cellulase activity reached 0. 64 U/mL when the strain was cultured in a medium that consisted of CMC, peptone and yeast extract. The optimal pH and the optimal temperature for the produced alkaline cellulase are 10. 0 and 400C respectively. The characters for cellulase production with strain ATCC 21833 and the produced cellulase are different from that of Bacillus strain N-4, proving these two organisms are different.