荧光寿命成像及其在生物医学中的应用-《深圳大学学报理工版》

文章信息/Info

作者:: 刘立新¹; 2; 3; 屈军乐³; 林子扬³; 陈丹妮³; 郭宝平³; 牛憨笨³; 1）中国科学院西安光学精密机械研究所西安710068；
2）中国科学院研究生院北京100864；
3）深圳大学光电子学研究所深圳 518060

Author(s):: LIU Li-xin¹; 2; 3; QU Jun-le³; GUO Bao-ping³; LIN Zi-yang³; CHEN Dan-ni³; and NIU Han-ben³; 1)Xi’an Institute of Optics and Precession Mechanics Chinese Academy of Sciences Xi’an 710068 P. R. China;
2)Graduate School of the Chinese Academy of Sciences Beijing 100864 P. R. China;
3)Institute of Optoelectronics Shenzhen University Shenzhen 518060 P. R. China

Keywords:: fluorescence lifetime; fluorescence lifetime imaging; synchroscan streak camera; time-correlated single photon counting; fluorescence resonance energy transfer; functional imaging; multispectral resolution

摘要:: 荧光寿命取决于荧光分子所处的微环境，通过对样品荧光寿命的测量和成像可以定量获取样品的功能信息.介绍荧光寿命成像(fluorescence lifetime imaging, FLIM)技术原理、实现途径、发展现状及其在生物医学中的应用‘提出一种基于皮秒扫描相机、梯度折射率微透镜阵列、棱镜分光光谱仪、飞秒钛宝石激光器和荧光显微物镜的新型五维荧光显微成像技术，该系统具有多光谱分辨功能以及很高的时间和空间分辫率，能够实现三维多光子荧光强度、光谱分辫强度和寿命测量，将在高通量生化分析、组织鉴别、胞内生理学和荧光共振能量转移(fluorescence resonance energy transfer, FRET)等领域获得广泛应用.

Abstract:: Fluorescence lifetime is dependent on the microenvironment of the fluorophore and its measurement and imaging can provide functional information about the sample. The principle，methods，current developments and bio-medical applications of fluorescence lifetime imaging( FILM) were discussed. A novel 5-D fluorescence microscopy that was the combination of a picosecond streak camera，a. gradient-index microlens array，a prism spectrophotometer, a femtosecond Ti: Sapphire laser and a fluorescence microscope is presented. The system is versatile with multispectral capability，high temporal and spatial resolution and can be used to make 3-dimensitional multiphoton fluorescence intensity, spectrally resolved intensity and lifetime measurements with a single detector. Potential applications of this system include multiwell imaging, tissue discrimination, intracellular physiology and fluorescence resonance energy transfer imaging.