[1]刘森林.两种体系中酶促合成(R)-苯乙氰醇的对比研究[J].深圳大学学报理工版,2005,22(1):80-84.
 LIU Sen-lin.Comparative study on the asymmetric synthesis of (R)-mandelonitrile catalysed by (R)-oxynitrilase in two systems[J].Journal of Shenzhen University Science and Engineering,2005,22(1):80-84.
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两种体系中酶促合成(R)-苯乙氰醇的对比研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第22卷
期数:
2005年1期
页码:
80-84
栏目:
生命科学
出版日期:
2005-01-30

文章信息/Info

Title:
Comparative study on the asymmetric synthesis of (R)-mandelonitrile catalysed by (R)-oxynitrilase in two systems
文章编号:
1000-2618(2005)01-0080-05
作者:
刘森林
深圳大学生命科学学院, 深圳 518060
Author(s):
LIU Sen-lin
College of Life Science Shenzhen University, Shenzhen 518060 P .R. China
关键词:
微水相水/有机溶剂双相醇腈酶 (R)-苯乙氰醇
Keywords:
organic solvent system water/organic solvent biphase (R)-oxynitrilase (R)-mandelonitrile
分类号:
Q 55/O 643
文献标志码:
A
摘要:
在微水相和水/有机溶剂双相两种反应体系中,利用杏仁(R)-醇腈酶催化苯甲醛与HCN不对称合成(R)-苯乙氰醇,对比探讨了有机溶剂、水、pH值和温度等对两种反应体系中醇腈酶反应的影响.结果表明,上述因素对两种体系中醇腈酶促不对称合成(R)-苯乙氰醇反应均有显著影响.在常温(25℃)下,微水相中酶反应的转化率和产物光学纯度优于水/有机溶剂双相中的相应值但水/有机溶剂双相中酶反应速度较微水相中快得多.异丙醚是两种反应体系中最好的有机溶剂,两种体系中最佳反应温度均为0~5℃.在低温(0~5℃)下,微水相和水/有机溶剂双相中平衡转化率和产物的光学纯度均高达99%以上.
Abstract:
The asymmetric synthesis of (R)-mandelonitrile from benzaldehyde and hydrogen cyanide catalysed by (R)-oxynitrilase in organic solvent and in water/organic solvent biphase was studied. The effects of organic solvents, water, pH value and reaction temperature on the reaction in two systems were analized systematically. It was found that, at room temperature (25℃), the substrate conversion and product enantiomeric excess in organic solvent system were higher than those in water/organic solvent biphase, but the reaction rate in water/organic solvent biphase was much faster than that in organic solvent. Among all organic solvents examined, diisopropyl ether was found to be the best organic solvent for the reaction. The optimum reaction temperature was 0~5℃ lost inboth systems. At low temperature (0~5℃), either substrate conversion or product 99% were over 99% in both systems.
更新日期/Last Update: 2015-10-16