[1]余玉雯,孙海丹,郑易之,等.大豆耐盐相关基因的分离及其功能鉴定[J].深圳大学学报理工版,2004,21(4):324-330.
　YU Yu-wen,SUN Hai-dan,ZHENG Yi-zhi,et al.Isolation and characterization of genes related to salt-tolerance in soybean[J].Journal of Shenzhen University Science and Engineering,2004,21(4):324-330.
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大豆耐盐相关基因的分离及其功能鉴定()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第21卷
期数:
2004年4期
页码:
324-330
栏目:
生命科学
出版日期:
2004-10-30

文章信息/Info

Title:
Isolation and characterization of genes related to salt-tolerance in soybean
文章编号:
1000-2618(2004)04-0324-07
作者:
余玉雯; 孙海丹; 郑易之; 兰英; 胥顺; 唐玉林; 麻晓亮
深圳大学生命科学学院,深圳市微生物基因工程重点实验室，深圳 518060
Author(s):
YU Yu-wen;  SUN Hai-dan;  ZHENG Yi-zhi;  LAN Ying;  XU Shun;  TANG Yu-lin;  and MA Xiao-liang
College of Life Science, Shenzhen University，Shenzhen 518060, P. R. China
关键词:
大肠杆菌; 功能筛选; 耐盐基因; 大豆
Keywords:
Escherichia coli;  functional screening;  salt-tolerant gene;  soybean
分类号:
Q 781
文献标志码:
A
摘要:
利用大肠杆菌功能表达体系,从大豆开花40 d未成熟种子的cDNA表达文库中筛选获得了11个耐盐相关cDNA克隆.对其中3个克隆的插入片段进行测序.结果显示,其中的Gm1013为一尚未报道的新基因片段; GmRPS25-2编码的蛋白质序列与西红柿40S核糖体蛋白S25有91%的同源性;GmAIP-2编码的蛋白质序列与大豆根铝诱导蛋白SALI3-2有97%的同源性.其中GmAIP-2可表达分子量为30?8kD的多肽. pET-GmAIP-2转化后的大肠杆菌在含800 mmol/L NaCl和700 mmol/L KCl的液体培养基中生长状况明显好于对照菌,即前者表现出较短的生长迟滞期和较高的生长量.
Abstract:
11 salt-tolerant cDNA colonies were isolated from a cDNA library constructed from unmatured seeds of soybean by functional screening withE?coli?Sequencing of the inserts of 3 clolonies showed thatGm1013is an unknown new gene;GmRPS25-2is homologous to a gene encoding a 40S ribosomal protein S25 in tomato with 91%identity andGmAIP-2is homologous to the gene encoding an aluminium-induced protein SALI3-2 in soybean with 97%identity.GmAIP-2could express a protein of 30?8kD. The transformant containing pET-GmAIP-2 displayed enhanced salt tolerance in liquid mediums with 800 mmol/L NaCl or 700 mmol/L KCl when compared with the control strain, and showed the shorter lag period and higher bacterial quantity.

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更新日期/Last Update: 2015-11-06