[1]刘士德,丘劲,张建华,等.菌多糖对Balb c 3T3、Hela、HEK-293细胞增殖的影响[J].深圳大学学报理工版,2003,20(3):58-63.
 LIU Shi-de,QIU Jin,ZHANG Jian-hua and XING Miao.The Effect of Polysaccharides from Microbe on the Proliferation of Balb/c 3T3, Hela and HEK-293 Cells[J].Journal of Shenzhen University Science and Engineering,2003,20(3):58-63.
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菌多糖对Balb c 3T3、Hela、HEK-293细胞增殖的影响()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第20卷
期数:
2003年3期
页码:
58-63
栏目:
生命科学
出版日期:
2003-09-30

文章信息/Info

Title:
The Effect of Polysaccharides from Microbe on the Proliferation of Balb/c 3T3, Hela and HEK-293 Cells
文章编号:
1000-2618(2003)03-0058-06
作者:
刘士德丘劲张建华邢苗
深圳大学生命科学学院, 深圳 518060
Author(s):
LIU Shi-de QIU Jin ZHANG Jian-hua and XING Miao
College of Life Science Shenzhen University, Shenzhen 518060, P .R .China
关键词:
菌多糖MTT细胞增殖
Keywords:
polysaccharide MTT proliferation
分类号:
Q 253
文献标志码:
A
摘要:
用苯酚法提取JM109大肠杆菌、O157大肠杆菌、霍乱弧菌、伤寒杆菌及多头绒泡菌的菌多糖,用MTT法检测菌多糖在体外对Balb/c 3T3 (鼠成纤维细胞)、Hela (人上皮细胞) 和HEK-293 (人胚肾细胞) 增殖的影响.结果表明,菌多糖在低质量浓度(低于40mg/L)时抑制Hela细胞和HEK-293细胞的增殖,但促进Balb/c 3T3细胞的增殖;菌多糖超过一定质量浓度(80mg/L)时,对Balb/c 3T3细胞的增殖作用和对Hela、HEK-293 细胞增殖的抑制作用趋于饱和.
Abstract:
The effect of polysaccharides on the proliferation of Balb/c 3T3, Hela and HEK-293 cells was determined by MTT. The polysaccharides were extracted from JM109 E.coli, O157 E. coli,vibrio cholerae, typhoidin and Physarum polycephalum by phenol. The results showed that low concentration polysaccharides inhibit the proliferation of Hela and HEK-293 cells, but facilitate the proliferation of Balb/c 3T3 cells. The effect on the proliferation of Balb/c 3T3, Hela and HEK-293 cells reaches a constant value for polysaccharides with their concentration exceeding 80 mg/L.
更新日期/Last Update: 2015-11-13