[1]王晓东,姜咏,陈民盛.后肢悬吊后大鼠比目鱼肌的蛋白质组学解析[J].深圳大学学报理工版,2010,27(4):502-504.
 WANG Xiao-dong,JIANG Yong,and CHEN Min-sheng.Proteomics analysis on rats soleus muscle fibers following hindlimb suspension[J].Journal of Shenzhen University Science and Engineering,2010,27(4):502-504.
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后肢悬吊后大鼠比目鱼肌的蛋白质组学解析()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第27卷
期数:
2010年4期
页码:
502-504
栏目:
生命科学
出版日期:
2010-10-31

文章信息/Info

Title:
Proteomics analysis on rats soleus muscle fibers following hindlimb suspension
文章编号:
1000-2618(2010)04-0502-03
作者:
王晓东姜咏陈民盛
深圳大学师范学院,深圳518060
Author(s):
WANG Xiao-dongJIANG Yongand CHEN Min-sheng
Normal College, Shenzhen University, Shenzhen 518060, P.R.China
关键词:
蛋白质组学比目鱼肌肌肉萎缩后肢悬吊差异表达蛋白
Keywords:
proteomicssoleus musclemuscle atrophyhindlimb suspensiondifferential expressed protein
分类号:
R 337;R 852.22
文献标志码:
A
摘要:
分析Wistar 和Wistar Hannover 大鼠经16天后肢悬吊后,比目鱼肌的全单一肌纤维形态变化与蛋白表达差异.发现随着后肢悬吊,Wistar Hannover 大鼠的比目鱼肌发生很大变化.尽管两种大鼠肌纤维长度未发生变化,但全单一肌纤维的横断面积,肌核数量等减少度Wistar Hannover大鼠相对较大.后肢悬吊前后的蛋白质组学解析结果表明,热休克蛋白27 (HSP27)在两个种别大鼠的比目鱼肌内表现有差异.
Abstract:
Effects of 16 days of hindlimb suspension on the characteristics of single soleus muscle fiber were compared between male Wistar and Wistar Hannover rats. The greater effects of unloading were noted in Wistar Hannover rats. The unloading-related reductions of muscle weight and fiber cross-sectional area versus the pre-suspension levels were greater than Wistar rats. Total number of myonuclei in control situation was greater in Wistar Hannover rats,but the more numbers of myonuclei were decreased following unloading. Responses of myonuclear domain levels were similar. The numbers of both quiescent and mitotic active satellite cells in control situation were greater in Wistar rats.But the magnitude of the unloading-related decrease was identical for Wistar Hannover and Wistar rats. Although the level of heat shock protein 27 (HSP27) expression in Wistar rats was decreased by unloading,de novo appearance of HSP27 was noted in Wistar Hannover rats. It is suggested that greater responses of soleus muscle fibers of Wistar Hannover than Wistar rats may be related to the different expression of protein,although the precise mechanism is still unclear.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-06-17;修回日期:2010-07-13
基金项目:留学回国人员科研启动基金资助项目(教外司留2010-609)
作者简介:王晓东(1972-),男(汉族),河北省河间市人,深圳大学副教授、博士.E-mail:Xiaodong19720530@yahoo.com.cn
更新日期/Last Update: 2010-11-09