[1]夏立新,闫浩,汤慕瑾,等.花生过敏原Ara h2与Ara h6的生物信息学比较研究[J].深圳大学学报理工版,2010,27(2):241-246.
 XIA Li-xin,YAN Hao,TANG Mu-jin,et al.Bioinformatics comparison of peanut allergen Ara h2 and Ara h6[J].Journal of Shenzhen University Science and Engineering,2010,27(2):241-246.
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花生过敏原Ara h2与Ara h6的生物信息学比较研究()
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《深圳大学学报理工版》[ISSN:1000-2618/CN:44-1401/N]

卷:
第27卷
期数:
2010年2期
页码:
241-246
栏目:
生物医学工程
出版日期:
2010-04-30

文章信息/Info

Title:
Bioinformatics comparison of peanut allergen Ara h2 and Ara h6
文章编号:
1000-2618(2010)02-0241-06
作者:
夏立新1闫浩2汤慕瑾3朱海3刘志刚1
1)深圳大学医学院 呼吸疾病国家重点实验室深圳大学变态反应分室,深圳 518060
2)深圳大学生命科学学院,深圳 518060;3.深圳出入境检验检疫局,深圳 518045
Author(s):
XIA Li-xin1YAN Hao2TANG Mu-jin3ZHU Hai3and LIU Zhi-gang1
1)College of Medicine,State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University,Shenzhen University,Shenzhen 518060,P.R.China
2)College of Life Sciences,Shenzhen University,Shenzhen 518060,P.R.China
3)Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518045,P.R.China
关键词:
生物信息学免疫花生过敏原同源建模Ara h2过敏原Ara h6过敏原抗原表位交叉反应低致敏原疫苗
Keywords:
bioinformaticsimmunologyallergenhomology modelingAra h2Ara h6epitopecrossreactivity hypoallergen vaccine
分类号:
R 392.8;Q 811.4
文献标志码:
A
摘要:
运用生物信息学技术比较Ara h2和Ara h6从一级结构到三级结构的异同.结果发现,Ara h2含有一段独有的序列(60~73),其中包括Ara h2三个主要IgE抗原表位中的两个.以Ara h6为模板进行同源建模获得了Ara h2的立体结构,与Ara h6的结构叠加拟合后,该结构多出一段由蛋白环连接的反向平行β-折叠(58~72),其伸展结构同样包含上述两个IgE表位的蛋白序列.探讨从Ara h2和Ara h6的一级结构到三级结构产生免疫学活性差异的原因,为研究花生过敏机制及设计低致敏原疫苗奠定基础.
Abstract:
The allergens Ara h2 and Ara h6 are the most clinically relevant allergens of peanut allergies.Ara h2 can completely inhibit the IgE binding ability of Ara h6 while Ara h6 can only partially inhibit IgE epitope of Ara h2.Comparison between the primary and tertiary structures of Ara h2 and Ara h6 is carried out for the exploration of this mechanism.Ara h2 contains a unique fragment (from 60 to 73) which includes two of the three major linear IgE epitopes of Ara h2.A 3-D structure of Ara h2 is obtained by homology modeling with Ara h6 as the template.When the structure of Ara h2 and Ara h6 are superposed,an extra outstretched anti-parallel β-sheet linked a loop (from 58 to 72) is found within the structure of Ara h2.It also contains the sequence encoding the above-mentioned two IgE epitopes.This study gives a explanation for the difference of Ara h2 and Ara h6 by comparison of primary and tertiary structures of Ara h2 and Ara h6. The explanation lays down the foundation for understanding of the mechanisms of peanut allergies and future development of hypoallergic vaccines.

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备注/Memo

备注/Memo:
收稿日期:2009-12-01;修回日期:2010-01-30
基金项目:国家自然科学基金资助项目(30871752);深圳大学创新团队基金资助项目(200904)
作者简介:夏立新(1975-),男(汉族),湖北省阳新县人,深圳大学副教授、博士.E-mail:xialixin@126.com
通讯作者:刘志刚(1959-),男(汉族),深圳大学教授、博士生导师.E-mail:lzg@szu.edu.cn
更新日期/Last Update: 2010-05-08