|Table of Contents|

Preparation and performance of hydroxyapatite-graphene oxide composite microspheres(PDF)

Journal of Shenzhen University Science and Engineering[ISSN:1000-2618/CN:44-1401/N]

Issue:
2022 Vol.39 No.4(363-488)
Page:
447-455
Research Field:
Chemistry & Chemical engineering

Info

Title:
Preparation and performance of hydroxyapatite-graphene oxide composite microspheres
Author(s):
YUAN Qiuhua SHI Xin WU Wenshan DAI Xiaoyi ZHONG Junxi YANG Yuan JIAN Youliang LI Ruilong and WANG Tao
College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518071, Guangdong Province, P. R. China
Keywords:
biomedical materials composite microspheres hydroxyapatite (HA) graphene oxide (GO) template method curcumin drug loading properties
PACS:
R318.08
DOI:
10.3724/SP.J.1249.2022.04447
Abstract:
Drug-loaded microspheres have been widely studied and applied in biomedical materials, but there are still some open problems such as low drug loading and sudden release. In order to solve such problems, hydroxyapatite-graphene oxide (HA-GO) composite microspheres were prepared by using hydroxyapatite (HA) and graphene oxide (GO). Firstly, spherical calcium carbonate-graphene oxide (CaCO3-GO) composites were synthesized by hard template method. Then, spherical hollow HA-GO composite microspheres were successfully prepared by a method of hydrothermal-assisted ion exchange. The effect of different synthesis conditions on the prepared HA-GO composites was studied. Through a series of measurements, such as X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Raman infrared spectroscopy, field emission scanning electron microscope (FESEM), ultraviolet visible spectrophotometer (UV-VIS), etc., the prepared samples were analyzed and characterized. The drug-loading performance of the microspheres was tested by using curcumin as the drug-loading model, and the drug-loading performance was evaluated by two indicators of encapsulation efficiency and drug-loading capacity. At the same time, the cytotoxicity test of the microsphere sample materials was also performed. The research results show that the reactant concentration of the system and the hydrothermal reaction time greatly affect the forming effect of the composite microspheres. When the initial reactant concentration is 0.3 mol/L and the hydrothermal reaction time is 6 h, hollow HA-GO composite microspheres with good morphology can be prepared, with a particle size from 5.1 μm to 7.7 μm and a pore size of about 40 nm. At the same time, it was found that the spherical structure can improve the drug loading, the drug encapsulation efficiency was (20.90 ± 0.31)%, and the drug loading was (2.95 ± 0.19)%. This shows that HA-GO composite microspheres have good medical application value.

References:

[1] SUZUKI O, SHIWAKU Y, HAMAI R. Octacalcium phosphate bone substitute materials: comparison between properties of biomaterials and other calcium phosphate materials [J]. Dental Materials Journal, 2020, 39(2): 187-199.
[2] SOON Y M, SHIN K H, KOH Y H, et al. Fabrication and compressive strength of porous hydroxyapatite scaffolds with a functionally graded core/shell structure [J]. Journal of the European Ceramic Society, 2011, 31(1/2): 13-18.
[3] MESKINFAM M, BERTOLDI S, ALBANESE N, et al. Polyurethane foam/nano hydroxyapatite composite as a suitable scaffold for bone tissue regeneration [J]. Materials Science & Engineering C-Materials for Biological Applications, 2018, 82: 130-140.
[4] KATARZYNA S, AMANDA B, MARCIN P, et al. Electrochemical properties and bioactivity of hydroxyapatite coatings prepared by MEA/EDTA double-regulated hydrothermal synthesis [J]. Electrochimica Acta, 2019, 298: 685-693.
[5] WU Yanhong, CHEN Diyun KONG Lingjun, et al. Rapid and effective removal of uranium (VI) from aqueous solution by facile synthesized hierarchical hollow hydroxyapatite microspheres [J]. Journal of Hazardous Materials, 2019, 371: 397-405.
[6] NURETTIN S, COSKUN S, SELIN S, et al. Porous and modified HA particles as potential drug delivery systems [J]. Microporous and Mesoporous Materials, 2012, 155: 124-130.
[7] WU Yaping, CHEN Jing, CHEN Gang, et al. Convenient synthesis of hydroxyapatite-coated ferroferric oxide microspheres by hydrothermal method [J]. Materials Letters, 2019, 253: 218-221.
[8] AKINDOYO J O, BEG M D H, GHAZALI S, et al. Impact modified PLA-hydroxyapatite composites: thermomechanical properties [J]. Composites Part A: Applied Science and Manufacturing, 2018, 107: 326-333.
[9] DEVI G V Y, PRABHU A, ANIL S, et al. Preparation and characterization of dexamethasone loaded sodium alginate-graphene oxide microspheres for bone tissue engineering [J]. Journal of Drug Delivery Science and Technology, 2021, 64: 9.
[10] GAO Junguo, ZHAO He, SUN Weifeng. Molecular dynamics simulation study of parallel orientation structure and gas transport in graphite-nanoplatelet/polyethylene composites [J]. Materials Today Communications, 2017, 13: 57-64.
[11] MINA K, MOHAMMAD A H F, MAJID M, et al. Development of curcumin-loaded gemini surfactant nanoparticles: Synthesis, characterization and evaluation of anticancer activity against human breast cancer cell lines [J]. Phytomedicine, 2019, 57:183-190.
[12] TABANELLI R, BROGI S, CALDERONE V. Improving curcumin bioavailability: current strategies and future perspectives [J]. Pharmaceutics, 2021, 13(10): 37.
[13] MATLOUB Z, HASSAN Z. HSA-curcumin nanoparticles: a promising substitution for curcumin as a cancer chemoprevention and therapy [J]. Daru-Journal of Pharmaceutical Sciences, 2020, 28(1): 209-219.
[14] DIZAJ S M, ALIPOUR M, ABDOLAHINIA E D, et al. Curcumin nanoformulations: beneficial nanomedicine against cancer [J]. Phytotherapy Research, 2022, 36(3): 1156-1181.
[15] PRASADS, DUBOURDIEU D, SRIVASTAVA A, et al. Metal-curcumin complexes in therapeutics: an approach to enhance pharmacological effects of curcumin [J]. International Journal of Molecular Sciences, 2021, 22(13): 24.
[16] GUO Xia, LI Wenfeng, WANG Heping, et al. Preparation, characterization, release and antioxidant activity of curcumin-loaded amorphous calcium phosphate nanoparticles [J]. Journal of Non-Crystalline Solids, 2018, 500: 317-325.
[17] ZHENG Yaxin, LIU Xun, MA Yongjun, et al. Controlled synthesis of hydroxyapatite microspheres with hierarchical structure and high cell viability [J]. Materials Letters, 2017, 195: 18-21.
[18] TANUSHREE B, BONAMALI P, SATNAM S. Hollow chitosan nanocomposite as drug carrier system for controlled delivery of ramipril [J]. Chemical Physics Letters, 2018, 706: 465-471.
[19] 国家药典委员会.中华人民共和国药典[M]. 2005版2部. 北京:化学工业出版社,2005.
National Pharmacopoeia Commission. The Pharmacopoeia of the People’s Republic of China [M]. 2005 ed. Two. Beijing: Chemical Industry Press, 2005.(in Chinese)
[20] 马在强. 碳酸钙晶型调控及机理研究[D].大庆:东北石油大学,2019.
MA Zaiqiang. Regulation and mechanism of calcium carbonate crystal form [D]. Daqing: Northeast Petroleum University, 2019.(in Chinese)
[21] 袁秋华,陈泽汇,万磊,等. 锶掺杂羟基磷灰石-石墨烯复合材料制备与表征[J]. 深圳大学学报理工版, 2020,37(3):298-304.
YUAN Qiuhua, CHEN Zehui, WANG Lei, et al. Preparation and characterization of strontium-doped hydroxyapatite-graphene composites [J]. Journal of Shenzhen University Science and Engineering, 2020, 37(3): 298-304.(in Chinese)
[22] 袁秋华,石鑫,梁进仁,等. 铈锌共掺杂HA-GP复合物合成及抗菌性研究[J]. 深圳大学学报理工版, 2021,38(3):280-286.
YUAN Qiuhua, SHI Xing, LIANG Jinren, et al. Synthesis and antibacterial properties of Ce-Zn co-doped HA-GP complexes [J]. Journal of Shenzhen University Science and Engineering, 2021, 38(3): 280-286.(in Chinese)
[23] GUO Yajun, WANG Yingying, CHEN Ting, et al. Hollow carbonated hydroxyapatite microspheres with mesoporous structure: Hydrothermal fabrication and drug delivery property [J]. Materials Science and Engineering C, 2013, 33(6): 3166-3172.
[24] MILOVAC D, WEIGAND I, KOVACIC M, et al. Highly porous hydroxyapatite derived from cuttlefish bone as TiO2 catalyst support [J]. Processing and Application of Ceramics, 2018, 12(2): 136-142.
[25] El-MORSY M A, AWWAD N S, IBRAHIUM H A, et al. Optimizing the mechanical and surface topography of hydroxyapatite/Gd2O3/graphene oxide nanocomposites for medical applications [J]. Journal of Saudi Chemical Society, 2022, 26(3): 14.

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