|Table of Contents|

Analysis of the initial response pattern of Chlamydomonas reinhardtii to methyl jasmonate (MeJA) treatment(PDF)

Journal of Shenzhen University Science and Engineering[ISSN:1000-2618/CN:44-1401/N]

Issue:
2022 Vol.39 No.3(237-362)
Page:
237-244
Research Field:

Info

Title:
Analysis of the initial response pattern of Chlamydomonas reinhardtii to methyl jasmonate (MeJA) treatment
Author(s):
JIA Bin LAN Chengxiang and LI Xiangyu
College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Guangdong Technology Research Center for Marine Algal Bioengineering, Shenzhen University, Shenzhen 518071, Guangdong Province, P. R. China
Keywords:
metabolic engineering metabolic regulate Chlamydomonas reinhardtii methyl jasmonate isoprenoids 2-C-methnatureyl-D-rythritol-4-phosphate (MEP) pathway photosynthetic efficiency analysis transcriptional analysis
PACS:
Q946
DOI:
10.3724/SP.J.1249.2022.03237
Abstract:
As a chemical elicitor of plants, methyl jasmonate (MeJA) is a regulator of many secondary metabolisms in plants, which participates in the regulation of isoprenoid metabolism and has an important impact on the synthesis of isoprenoids in Chlamydomonas reinhardtii (C. reinhardtii). In order to explore how C. reinhardtii responds rapidly to MeJA treatment, the physiological parameters such as growth rate and photosynthetic efficiency of C. reinhardtii under MeJA treatment were measured. The changes in the expression levels of key genes in the 2-C-methnatureyl-D-rythritol-4-phosphate (MEP) pathway and in the carotenoid and sterol synthesis pathway within 24 hours of MeJA treatment were focused. The results showed that MeJA treatment dramatically reduced biomass accumulation and photosynthetic activity of C. reinhardtii, prominently activated the expression of key genes in MEP pathway and sterol synthesis, inhibited key genes of carotenoid synthesis pathway. The results at the transcriptional and physiological levels suggested that the influence of MeJA on C. reinhardtii was rapid and dose-dependent. This study provides a theoretical reference for exploring the mechanism of isoprene synthesis and metabolism in microalgae.

References:

[1] 罗秋兰,王潮岗,胡章立. 莱茵衣藻APC/C复合物基因家族及CrCDC20表达谱分析[J].深圳大学学报理工版,2018,35(2):172-178.
LUO Qiulan, WANG Chaogang, HU Zhangli. Bioinformatical analysis of genes encoding APC/C complex and the expression profiles analysis of CrCDC20 in Chlamydomanos reinhardtii [J]. Journal of Shenzhen University Science and Engineering, 2018, 35(2): 172-178.(in Chinese)
[2] PERIN G, BELLAN A, BERNARDI A, et al. The potential of quantitative models to improve microalgae photosynthetic efficiency [J]. Physiologia Plantarum, 2019, 166(1): 380-391.
[3] TREVES H, SIEMIATKOWSKA B, L UZAROWSKA U, et al. Multi-omics reveals mechanisms of total resistance to extreme illumination of a desert alga [J]. Nature Plants, 2020, 6(8): 1031-1043.
[4] VILLARO S, CIARDI M, MORILLAS-ESPANA A, et al. Microalgae derived astaxanthin: research and consumer trends and industrial use as food [J]. Foods, 2021, 10(10):2303.
[5] 娄素琳,朱秀兰,曾志勇,等.盐藻microRNAs高通量测序和生物信息学分析[J].深圳大学学报理工版,2018,35(4):331-333.
LOU Sulin, ZHU Xiulan, ZENG Zhiyong, et al. Bioinformatics analysis of Dunaliella microRNAs by high-throughput sequencing [J]. Journal of Shenzhen University Science and Engineering, 2018, 35(4): 331-333.(in Chinese)
[6] LEONG Y K, CHEN C Y, VARJANI S, et al. Producing fucoxanthin from algae - recent advances in cultivation strategies and downstream processing [J]. Bioresource Technology, 2022, 344(Pt A): 126170.
[7] 黄瑛,李小连,殷剑波,等. 3个基因共调控下莱茵衣藻的转录组分析[J].深圳大学学报理工版,2021,38(2):194-200.
HUANG Ying, LI Xiaolian, YIN Jianbo, et al. Transcriptome analysis of Chlamydomonas reinhardtii under the co-regulation of three genes [J]. Journal of Shenzhen University Science and Engineering, 2021, 38(2): 194-200.(in Chinese)
[8] WICHMANN J, BAIER T, WENTNAGEL E, et al. Tailored carbon partitioning for phototrophic production of (E)-alpha-bisabolene from the green microalga Chlamydomonas reinhardtii [J]. Metabolic Engineering, 2018, 45: 211-222.
[9] LAUERSEN K J, WICHMANN J, BAIER T, et al. Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii [J]. Metabolic Engineering, 2018, 49: 116-127.
[10] LAUERSEN K J, BAIER T, WICHMANN J, et al. Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii [J]. Metabolic Engineering, 2016, 38: 331-343.
[11] EINHAUS A, BAIER T, ROSENSTENGEL M, et al. Rational promoter engineering enables robust terpene production in microalgae [J]. ACS Synthetic Biology, 2021, 10(4): 847-856.
[12] RAMIREZ-ESTRADA K, VIDAL-LIMON H, HIDALGO D, et al. Elicitation, an effective strategy for the biotech-nological production of bioactive high-added value compounds in plant cell factories [J]. Molecules, 2016, 21(2): 182.
[13] VITHANA M D, SINGH Z, JOHNSON S K. Regulation of the levels of health promoting compounds: lupeol, mangiferin and phenolic acids in the pulp and peel of mango fruit: a review [J]. Journal of the Science of Food and Agriculture, 2019, 99(8): 3740-3751.
[14] CHEN Yue, WANG Yunzhu, L?(Lyu) Ping, et al. Comparative transcriptomic analysis reveal the regulation mechanism underlying MeJA-induced accumulation of alkaloids in Dendrobium officinale [J]. Journal of Plant Research, 2019, 132(3): 419-429.
[15] COMMAULT A S, FABRIS M, KUZHIUMPARAMBIL U, et al. Methyl jasmonate treatment affects the regulation of the 2-C-methyl-D-erythritol 4-phosphate pathway and early steps of the triterpenoid biosynthesis in Chlamydomonas reinhardtii [J]. Algal Research, 2019, 39: 11.
[16] COMMAULT A S, KUZHIUMPARAMBIL U, HERDEAN A, et al. Methyl jasmonate and methyl-beta-cyclodextrin individually boost triterpenoid biosynthesis in Chlamydomonas reinhardtii UVM4 [J]. Pharmaceuticals, 2021, 14(2): 12.
[17] LEE J-E, CHO Y U, KIM K H, et al. Distinctive metabo-lomic responses of Chlamydomonas reinhardtii to the chemical elicitation by methyl jasmonate and salicylic acid [J]. Process Biochemistry, 2016, 51(9): 1147-1154.
[18] ZHANG Luoyan, XING Zhaotian, CHEN Liqian, et al. Comprehensive time-course transcriptome and co-ex-pression network analyses identify salt stress responding mechanisms in Chlamydomonas reinhardtii strain GY-D55 [J]. Frontiers in Plant Science, 2022, 13: 828321.
[19] BEAUVAIS-FLUCK R, SLAVEYKOVA V I, COSIO C. Comparative study of Cu uptake and early transcriptome responses in the green microalga Chlamydomonas reinhardtii and the macrophyte Elodea nuttallii [J]. Environmental Pollution, 2019, 250: 331-337.
[20] XU Chen, WEI Hui, MOVAHEDI A, et al. Evaluation, characterization, expression profiling, and functional analysis of DXS and DXR genes of Populus trichocarpa [J]. Plant Physiology and Biochemistry, 2019, 142: 94-105.
[21] VACCARO M, OCAMPO BERNAL V, MALAFRONTE N, et al. High yield of bioactive abietane diterpenes in Salvia sclarea hairy roots by overexpressing cyanobacterial DXS or DXR genes [J]. Planta Medica, 2019, 85(11/12): 973-980.
[22] YOU M K, LEE Y J, KIM J K, et al. The organ-specific differential roles of rice DXS and DXR, the first two enzymes of the MEP pathway, in carotenoid metabolism in Oryza sativa leaves and seeds [J]. BMC Plant Biology, 2020, 20(1): 167.
[23] 杨杰,詹亚光,肖佳雷,等. 细胞色素P450在植物三萜和甾醇骨架修饰中的功能研究进展[J]. 中国科学:生命科学,2018,48(10):1065-1083.
YANG Jie, ZHAN Yaguang, XIAO Jialei, et al. Research progress on the function of cytochrome P450 in the modification of plant triterpenes and sterol backbones [J]. Science in China: Life Sciences, 2018, 48(10): 1065-1083.(in Chinese)

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