|Table of Contents|

Analysis of the initial response pattern of Chlamydomonas reinhardtii to methyl jasmonate (MeJA) treatment(PDF)

Journal of Shenzhen University Science and Engineering[ISSN:1000-2618/CN:44-1401/N]

Issue:
2022 Vol.39 No.3(237-362)
Page:
237-244
Research Field:

Info

Title:
Analysis of the initial response pattern of Chlamydomonas reinhardtii to methyl jasmonate (MeJA) treatment
Author(s):
JIA Bin;  LAN Chengxiang;  and LI Xiangyu
College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Guangdong Technology Research Center for Marine Algal Bioengineering, Shenzhen University, Shenzhen 518071, Guangdong Province, P. R. China
Keywords:
metabolic engineering;  metabolic regulate;  Chlamydomonas reinhardtii;  methyl jasmonate;  isoprenoids;  2-C-methnatureyl-D-rythritol-4-phosphate (MEP) pathway;  photosynthetic efficiency analysis;  transcriptional analysis
PACS:
Q946
DOI:
10.3724/SP.J.1249.2022.03237
Abstract:
As a chemical elicitor of plants, methyl jasmonate (MeJA) is a regulator of many secondary metabolisms in plants, which participates in the regulation of isoprenoid metabolism and has an important impact on the synthesis of isoprenoids in Chlamydomonas reinhardtii (C. reinhardtii). In order to explore how C. reinhardtii responds rapidly to MeJA treatment, the physiological parameters such as growth rate and photosynthetic efficiency of C. reinhardtii under MeJA treatment were measured. The changes in the expression levels of key genes in the 2-C-methnatureyl-D-rythritol-4-phosphate (MEP) pathway and in the carotenoid and sterol synthesis pathway within 24 hours of MeJA treatment were focused. The results showed that MeJA treatment dramatically reduced biomass accumulation and photosynthetic activity of C. reinhardtii, prominently activated the expression of key genes in MEP pathway and sterol synthesis, inhibited key genes of carotenoid synthesis pathway. The results at the transcriptional and physiological levels suggested that the influence of MeJA on C. reinhardtii was rapid and dose-dependent. This study provides a theoretical reference for exploring the mechanism of isoprene synthesis and metabolism in microalgae.

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