罗秋兰,王潮岗,胡章立.莱茵衣藻APC/C复合物基因家族及CrCDC20表达谱分析[J].深圳大学学报理工版,2018,35(No.2(111-220)):172-178.[doi:10.3724/SP.J.1249.2018.02172]
LUO Qiulan,WANG Chaogang,and HU Zhangli.Bioinformatical analysis of genes encoding APC/C complex and the expression profiles analysis of CrCDC20 in Chlamydomanos reinhardtii[J].Journal of Shenzhen University Science and Engineering,2018,35(No.2(111-220)):172-178.[doi:10.3724/SP.J.1249.2018.02172]
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莱茵衣藻APC/C复合物基因家族及CrCDC20表达谱分析
罗秋兰1,2,3,4,王潮岗1,3,胡章立1,3

1)深圳大学生命与海洋科学学院,深圳市海洋生物资源与生态环境重点实验室,广东深圳 518060; 2)深圳大学光电工程学院,光电子器件与系统教育部/广东省重点实验室,广东深圳 518060; 3)深圳大学龙华生物产业创新研究院,深圳市海洋藻类生物工程技术研究中心,广东深圳 518060; 4)中国热带农业科学院热带生物技术研究所,海南海口 570100

微藻分子生物学; 生物柴油; 细胞周期; E3泛素连接酶; 进化树分析; 实时荧光定量反转录聚合酶链式反应

Bioinformatical analysis of genes encoding APC/C complex and the expression profiles analysis of CrCDC20 in Chlamydomanos reinhardtii
LUO Qiulan1,2,3,4, WANG Chaogang1,3, and HU Zhangli1,3

1)College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Sciences, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China2)College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, Guangdong 518060, Guangdong Province, P.R.China3)Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R China4)Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 570100, Hainan Province, P.R.China

molecular biology of microalgae; biodiesel; cell cycle; E3 ubiquitin ligase; phylogenetic analysis; real-time fluorescence quantitative reverse transcription polymerase chain reaction(rt-RT-qPCR)

DOI: 10.3724/SP.J.1249.2018.02172

备注

为揭示莱茵衣藻细胞周期调控机制,提高微藻生物量,通过生物信息学方法,对莱茵衣藻参与细胞周期控制的末期促进复合物(anaphase-promoting complex/cyclosome,APC/C)基因家族的性质进行系统分析,包括基因结构、蛋白质特性和进化树分析等. 结果显示,莱茵衣藻包含13个APC/C复合物编码基因,在13号染色体存在一个基因簇. 除了激活因子细胞分裂周期蛋白20(cell division cycle 20,CDC20)和CDC20同系蛋白1(CDC20 homolog 1,CDH1)相对保守外,莱茵衣藻APC/C复合物的其他亚基与高等植物来源的蛋白质保守性很低. 此外,利用实时荧光定量反转录聚合酶链式反应(real-time fluorescence quantitative reverse transcription polymerase chain reaction, rt-RT-qPCR)分析了莱茵衣藻CrCDC20基因在缺氮或缺硫下表达谱,发现CrCDC20基因在缺硫下上调表达,在缺氮下下调表达,说明CrCDC20参与莱茵衣藻的营养缺乏胁迫应答反应.

Chlamydomanos reinhardtii is a model microalgae for lipids production. There is a contradiction between lipids biosynthesis and biomass accumulation in C. reinhardtii. Revealing regulation mechanisms of cell cycle can prove a new direction for promoting biomass in C. reinhardtii. In this paper, the gene structures, protein characteristics, and phylogenetic tree of anaphase-promoting complex/cyclosome(APC/C)gene family involving in cell cycle control are analyzed through bioinformatics. Totally 13 genes of APC/C family are identified in C. reinhardtii, and one gene cluster is found in chromosome 13. The phylogenetic tree shows that APC/C proteios, except the activation factors CrCDC20 and CrCDH1 from C. reinhardtii, have low homologous with the proteios in higher plants. Meanwhile, the gene expression profiles of CrCDC20 under sulfur or nitrogen deficiency conditions are determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction(rt-RT-qPCR). The results show that CrCDC20 is up-regulate under sulfur deficiency but down-regulate under nitrogen deficiency, which indicates that CrCDC20 might involve in nutrition deficiency stresses.

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