深圳大学学报理工版

马轩,李盛本,莫蓓莘,等.拟南芥ago1-27突变体的RNA-seq分析[J].深圳大学学报理工版,2017,34(No.1(001-110)):27-32.[doi:10.3724/SP.J.1249.2017.01027]
Ma Xuan,Li Shengben,Mo Beixin,et al.RNA-seq analysis on Arabidopsis ago1-27 mutant[J].Journal of Shenzhen University Science and Engineering,2017,34(No.1(001-110)):27-32.[doi:10.3724/SP.J.1249.2017.01027]

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拟南芥ago1-27突变体的RNA-seq分析

马轩^1,2,李盛本³,莫蓓莘¹,曹晓风²,陈雪梅^1,4

1)深圳大学生命与海洋科学学院,广东深圳518060; 2)中国科学院遗传与发育生物学研究所,北京100101; 3)中国农业科学院深圳农业基因组研究所,广东深圳518120; 4)美国加利福尼亚大学河滨分校植物科学系, 加利福尼亚河滨CA 92521,美国

关键词：植物生物化学; miRNA; ago1-27基因; RNA-seq; 降解组; 靶基因; 拟南芥

RNA-seq analysis on Arabidopsis ago1-27 mutant

Ma Xuan^1,2, Li Shengben³, Mo Beixin¹, Cao Xiaofeng², and Chen Xuemei^1,4

Ma Xuan^1,2, Li Shengben³, Mo Beixin¹, Cao Xiaofeng², and Chen Xuemei^1,41)College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong Province, P.R.China2)Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Beijing 100101, P.R.China3)Shenzhen Institute of Agricultural Genomics, Chinese Academy of Agricultural Sciences,Shenzhen 518120, Guangdong Province, P.R.China4)Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, California 92521, USA

Keywords： phytobiochemistry; microribonucleic acid(miRNA); ago1-27; RNA-seq; degradome; target genes; Arabidopsis

DOI: 10.3724/SP.J.1249.2017.01027

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miRNA在植物生长发育和环境适应等方面起着极为重要的作用.本研究对拟南芥ago1-27突变体进行了RNA-seq,鉴定到几千个差异表达基因,并且miRNA靶基因倾向于在ago1-27中表达上调.ago1-27 RNA-seq结合降解组分析鉴定到新的miRNA靶基因,如miR396靶向半胱氨酸蛋白酶基因和miR167靶向编码AT-hook DNA结合蛋白的基因等.

miRNAs play important roles in plant development and adaptation to environment. We conduct the RNA-seq of Arabidopsis ago1-27 mutant and has identified thousands of differentially expressed genes. The study shows that miRNA targets tend to be upregulated in the ago1-27 mutant. New miRNA targets are identified by ago1-27 RNA-seq combined with degradome analysis. For examples, miR396 targets cysteine proteinase genes and miR167 targets a gene encoding AT-hook DNA-binding protein.