陈丽伊,刘国宝,郑易之.大豆PM1蛋白对脂质体及兔红细胞的稳定作用[J].深圳大学学报理工版,2016,(05):441-446.[doi:10.3724/SP.J.1249.2016.05441 ]
Chen Liyi,Liu Guobao,and Zheng Yizhi.Protection effect of soybean PM1 protein on liposome and the rabbit red blood cells[J].Journal of Shenzhen University Science and Engineering,2016,(05):441-446.[doi:10.3724/SP.J.1249.2016.05441 ]
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大豆PM1蛋白对脂质体及兔红细胞的稳定作用
陈丽伊,刘国宝,郑易之

深圳大学生命与海洋科学学院,深圳市微生物基因工程重点实验室, 广东深圳518060

胚胎晚期富集蛋白; PM1蛋白; 生物膜; 脂质体; 冷冻保护; 浊度法; 粒径法; 兔红细胞

Protection effect of soybean PM1 protein on liposome and the rabbit red blood cells
Chen Liyi,Liu Guobao, and Zheng Yizhi

Chen Liyi,Liu Guobao, and Zheng YizhiCollege of Life Sciences and Oceanography, Shenzhen University, Shenzhen Key Laboratory of Microbiology and Gene Engineering, Shenzhen 518060, Guangdong Province, P.R.China

late embryogenesis abundant(LEA)protein; PM1 protein; membrane; liposome; cryoprotection; turbidity assay; particle size assay; rabbit red blood cell

DOI: 10.3724/SP.J.1249.2016.05441

备注

利用浊度法、粒径法和相差显微镜技术研究反复冻融条件下大豆PM1蛋白(属于第4组胚胎晚期富集蛋白)对1-棕榈酰基-2-油酰基卵磷脂(1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC)脂质体的保护作用.结果发现,与未经冻融处理的POPC样品相比,经冻融胁迫POPC脂质体浊度升高、粒径增大,在GmPM1存在时,经冻融的POPC脂质体浊度和粒径增大程度明显降低,脂质体聚集物明显减少.在冻融条件下,GmPM1蛋白对兔血红细胞有明显保护作用.在低温胁迫中,GmPM1可能通过稳定细胞膜的磷脂起到保护细胞膜的作用.

We exploit approaches of a turbidity assay, a particle-size assay and a phase contrast microscopy to explore the protective effect of soybean protein PM1(LEA4)on 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine(POPC)liposome under the condition of repeated freezing and thawing. The results show that the turbidity and particle size of POPC liposome increased significantly after the treatment of freezing and thawing in contrast to those without freezing-thawing treatment; the increase of the turbidity and particle size of POPC liposome is reduced significantly after freezing-thawing treatment in the presence of PM1, and POPC aggregates decrease dsignificantly under a phase microscopy. The PM1 protein can maintain the stability of red blood cells from rabbit under the freezing-thawing condition.It can be inferred that PM1 could protect cell membranes by stabilizing its phospholipids under freezing-thawing stress.

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