深圳大学学报理工版

PEEK-HA生物复合材料对MG-63细胞的作用

1)深圳大学化学与化工学院,深圳 518060; 2)深圳大学生命科学学院,深圳 518060; 3)深圳市第六人民医院,深圳 518067

关键词：生物材料学; 复合材料; 骨替代材料; 聚醚醚酮-磺化聚醚醚酮-羟基磷灰石复合材料; 成骨MG- 63细胞; 黏附率; 细胞增殖; 细胞凋亡

Influence of PEEK-HA composites on MG-63 cells

Ni Zhuo^1, Wang Ying²,Wang Shuang¹, Liu Shide², Zhang Jianhua², Liu Xue³, and Ren Yinghua³

Ni Zhuo^1, Wang Ying²,Wang Shuang¹, Liu Shide², Zhang Jianhua², Liu Xue³, and Ren Yinghua³1)College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, P.R.China2)College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China3)Shenzhen Sixth People's Hospital, Shenzhen 518067, P.R.China

Keywords： biomaterials science; composites; bone substitute materials; polyetheretherketone-sulfnated polyetheretherketone-hydroxyapatite(PEEK-SPEEK-HA)composites; MG- 63 cells; cell adhesion; proliferation; apoptosis

DOI: 10.3724/SP.J.1249.2014.03258

备注

摘要

全文

图/表

参考文献

体外培养成骨MG- 63细胞, 通过倒置荧光显微镜和四甲基偶氮唑盐微量酶反应比色法,研究聚醚醚酮-磺化聚醚醚酮-羟基磷灰石(polyetheretherketone-sulfnated polyetheretherketone-hydroxyapatite, PEEK-SPEEK-HA)复合材料对细胞形态和增殖的影响.在PEEK-SPEEK-HA复合材料质量浓度为4 mg/mL时,复合材料对MG- 63细胞增殖作用最强,随浸提液质量浓度增大,材料对细胞增殖作用下降.材料质量浓度为4 mg/mL时,HA质量分数为20%的PEEK-SPEEK-HA复合材料对细胞的增殖作用较强. PEEK-HA和PEEK-SPEEK-HA复合材料的毒性评级为1级,无细胞毒性. SPEEK和HA能够改善复合材料对成骨MG- 63细胞的黏附作用,HA含量对细胞黏附作用的影响更显著. 扫描电镜结果表明MG- 63细胞在PEEK-SPEEK-HA材料上生长形态为三角形或长梭形,有伪足,伸展状态良好,细胞可以在材料缝隙处生长,表明成骨细胞能够在该材料表面黏附、伸展和生长. 将SPEEK引入PEEK-HA复合材料能抑制细胞凋亡.

Toxicity effects of PEEK-SPEEK-HA composite materials on the MG- 63 cells are studied in this paper. MTT results show that when the concentration of extracts increases, the cell proliferation decreases. In a concentration of 4 mg/mL,this material plays a significant role in cell proliferation. A concentration of 4 mg/mL has been selected to study the cell proliferation effect of different materials, and it is found that PEEK-SPEEK-HA(w(HA)=20%)composites have a stronger effect on cell proliferation than that of PEEK-HA(w(HA)=20%).The cytotoxicity of PEEK-SPEEK-HA and PEEK-HA composite is level 1, indicating no cytotoxicity. The cell adhesion rate increases with the increase of HA content in PEEK-HA composites, and scanning electron microscope(SEM)images show that MG- 63 cells can adhere, spread and grow on PEEK-SPEEK-HA surfaces. Apoptosis experiments show that PEEK-SPEEK-HA composite materials can inhibit apoptosis.

引言
1 材料和方法
2 结果与讨论
3 结语

[1] Sun Minglin,Hu Yunyu, Lei Wei, et al. Physical and chemical properties and extended release action of calcium phosphate cement/BMP composite bone graft material[J]. Acta Academiae Medicine Militaris Tertiae, 2001, 23(7): 800-802.(in Chinese)
[2] Jones D W. Characterization of experimental composite biomaterials[J]. Journal of Biomedical Materials Research,1996,33(2):89-92.
[3] Wang Jianying, Zhu Zhiguo, Sun Jiayao, et al. The study of PEEK artificial bone joint[J]. Chemical World, 2004, 45(1): 53-54.(in Chinese)
[4] Zhao Chun, Zhang Yulong. Polyetheretherketone[M].Beijing: Chemical Industry Press,2008.(in Chinese)
[5] Yan Pengtao, Li Wenke, Wang Yongpeng, et al.Preparation and properties of bioactivite coating on poly(ether ether ketone)composite[J]. Chemical Journal of Chinese Universities, 2013, 34(7): 1782-1787.(in Chinese)
[6] Yu Fangli, Zhou Yongqiang, Zhang Weike, et al. The present and prospect of research on hydroxyapatite bioceramic materials[J]. Ceramics, 2006(2): 7-12.(in Chinese)
[7] Bakar M,Cheong P,Khor K. Mechanical properties of injection molded hudroxyapatite-polyetheretherketone biomaterials[J]. Composites Science and Technology, 2003,63:421-425.
[8] Yu Shuchong, Hariraw K P, Kumarr R, et al. In vitro apatite formation and its growth kinetics on hydroxyapatite/polyetheretherketione biocomposites[J].Biomaterials,2005,26(15):2343-2352.
[9] Feng Xing,Sui Guoxin,Yang Rui.Mechanical properties and in vitro bioactivity of carbon fibre-reinforced polyetheretherketone-hydroxyapatite(PEEK-HA)composites for biomedical applications[J]. Chinese Journal of Materials Research, 2008, 22(1):18-25.(in Chinese)
[10] Ni Zhuo,Tian Shengli,Wang Ying,et al. PEEK/HA composite materials biocompatibility[J]. Orthopaedic Biomechanics Materials and Clinical Study, 2012,9(4):9-12.(in Chinese)
[11] Zhou Bing, Yu Chuang, Yang Yanhua,et al. Mechanical and Thermal Behaviour of CaCO3/PEEK Comopsites[J]. Chemical Journal of Chinese Universities,2004,25(7):1355-1358.(in Chinese)

₃

[12] Li Guangzhong, Zhang Wenyan, Han Jian, et al. Fabrication of TiO2 nanotube array film on near-β titanium alloy and its effects on osteoblast growth[J]. Rare Metal Materials and Engineering, 2010, 39(7): 1206-1209.(in Chinese)

₂

[13] Ni Zhuo,Liu Shide,Wang Ying,et al. Influence of 3T3 cells for PEEK-HA composite[J]. Journal of Shenzhen University Science and Engineering, 2013,30(4):392-397.(in Chinese)
[14] Xue Yuan, Wang Pei, Qi Qinghui, et al.The experimental study of the effects of icariin on increasing Smad4 mRNA level in MC3T3-E1 cell in vitro[J]. Chinese Journal of Orthopaedics, 2005, 25(2): 119-123.(in Chinese)
[15] Zhao Jiahui, Zhang Huaping, Wang Chunfang. Discussion in the detection of cell proliferation by MTT method[J]. Journal of Shanxi Medical University, 2007, 38(3): 262-263.(in Chinese)
[16] Zhou Hui, Liang Yu. Biosafety and biocompatibility of a variety of biological materials[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2009,13(38): 7559-7562.(in Chinese)
[17] Zhang Hui. The evaluation of the biocompatibility of nickel-free austenitic stainless stell using AnnexinV/PI and Hoechst33342/PI double staining method[D]. Beijing: Chinese Medical University, 2010.(in Chinese)
[18] GB/T16886.5—2003 Biological evaluation of medical devices-Part 5: In vitro cytotoxicity assays[S].(in Chinese)